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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in advertising cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been described. Two more splice variants had been claimed but in-depth nucleotide sequence facts is just not yet readily available.

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Blocking peptides are peptides that bind specially to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged with the antibody.

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This guide outlines further achievable optimization of cellular and immunoassay parameters to make certain the absolute best outcomes are...

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This information outlines further more feasible optimization of cellular and immunoassay parameters to guarantee the very best outcomes are...

Antibodies certain to the blocking peptide no more bind to your epitope over the concentrate on protein. By evaluating the staining within the blocked antibody vs . the antibody on your own, you can see which staining is particular.

Concentrate on Information and facts Cyclin E belongs for the hugely conserved cyclin loved ones, whose users are characterized by a remarkable periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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